1st European Top-Down Proteomics Symposium
Paris, February 12-14, 2019

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Tuesday, February 12, 2019
Opening of the 1st EuTDP Symposium
11:00 am-04:10 pm
CIS Auditorium
11:00 am

Registration and installation of posters

01:45 pm

Welcome and Opening

J. Chamot-Rooke
Mass Spectrometry for Biology Unit, Institut Pasteur, Paris, France 
02:00 pm

Opening Lecture: Profiling cellular-to-molecular diversity using electrophoretic cytometry

A. Herr
University of California, Berkeley, USA 
02:50 pm

Leveraging proteoforms and a growing community to determine the composition of the human proteome

N. Kelleher
Northwestern University, USA 
03:20 pm

The Top-Down Community

P. Danis, J. Chamot-Rooke
03:40 pm


Instrumentation and analytical strategies
04:10 pm-09:00 pm
CIS Auditorium
Chair: Shaw Jared, Pacific Northwest National Laboratory, USA
04:10 pm

Advances in Orbitrap mass spectrometry for top-down analysis

A. Makarov
Thermo Fisher Scientific, Germany 
04:40 pm

Enhancing middle-down and top-down protein and PTM characterization by mass spectrometry and new computational tools

O. Jensen
University of Southern Denmark, Denmark 
05:10 pm

Chromatographic strategies for top-down mass spectrometry of integral membrane proteins

J. Whitelegge
The Pasarow Mass Spectrometry Laboratory, University of California Los Angeles, Los Angeles, USA 
05:30 pm

Development of Liquid Extraction Surface Analysis Mass Spectrometry for Top-Down Protein Identification of ESKAPE Pathogens Growing on in vitro 3D Skin Models

J. Havlikova1-2, R.C. May3, I.B. Styles4-2, H.J. Cooper1
1School of Biosciences, University of Birmingham, Birmingham, United Kingdom 2EPSRC Centre for Doctoral Training in Physical Sciences for Health, University of Birmingham, Birmingham, United Kingdom 3Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom 4School of Computer Science, University of Birmingham, Birmingham, United Kingdom 
05:50 pm

And now a word from our sponsors!

06:00 pm

Round table: Sample preparation, intact protein separation, instrumentation, data analysis

L. Pasa-Tolic, J. Shaw
Pacific Northwest, National Laboratory, USA 
07:00 pm

Apéro Poster

Wednesday, February 13, 2019
Human Health
09:00 am-02:00 pm
CIS Auditorium
Chair: Tsybin Yury, Spectroswiss, Switzerland
09:00 am

A wealth of proteoforms exposed and quantified by hybrid mass spectrometry approaches

A. Heck
Utrecht University, The Netherlands 
09:50 am

Cutting-edge mass spectrometry methods for the multi-level structural characterization of Antibodies and Antibody Drug Conjugates

A. Beck
Pierre Fabre, France 
10:20 am

Sequence validation of three human IgG1 antibodies by Mass Spectrometry during an inter-Laboratory Study

A. Resemann1, S. Pengelley1, W. Evers1, E. Belau1, P. Schmit2, D. Suckau1
1Bruker Daltonik, Bremen, Germany 2Bruker France, Wissembourg, France 
10:40 am


11:10 am

Round table: Industrial and regulatory applications (i.e. antibody and biologics analysis)

Y. Tsybin1, A. Beck2
1CTDP 2Pierre Fabre, France 
12:10 pm

Lunch + Poster

Proteoform-centric Bioinformatics
02:00 pm-04:00 pm
CIS Auditorium
Chair: Pasa-Tolic Ljiljana, Pacific Northwest, National Laboratory, USA
02:00 pm

Implications of proteoforms in clinical chemistry (glyco)proteomics

Y. Van Der Burgt
Leiden University Medical Center, The Netherlands 
02:30 pm

UniProt protein resource and the proteoform atlas: linking proteoforms with the UniProt Knowledgebase

E. Alpi1, R. Leduc2, R. Fellers2, P. Thomas2, N. Kelleher2, T. Consortium For Top Down Proteomics3, M. Martin1, T. Uniprot Consortium1-4
1European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Cambridge, United Kingdom 2Northwestern University, Evanston, USA 3CTDP, Boston, USA 4Swiss Institute of Bioinformatics, Geneva, Switzerland and Protein Information Resource, Washington, USA 
02:50 pm

A proteoform centric paradigm for pathway and biological network analysis

L. Hernández Sánchez1-2, S. Johansson1-2, P. Njølstad1-3, H. Barsnes4-5, M. Vaudel1-2
1Diabetes Research Group, Department of Clinical Science, University of Bergen, Bergen, Norway 2Center for Medical Genetics and Molecular Medicine, Haukeland University Hospital, Bergen, Norway 3Department of Pediatrics, Haukeland University Hospital, Bergen, Norway 4Proteomics Unit, Department of Biomedicine, University of Bergen, Bergen, Norway 5Computational Biology Unit, Department of Informatics, University of Bergen, Bergen, Norway 
03:10 pm

And now a word from our sponsors!

03:20 pm


Technological Developments
04:00 pm-23:00
CIS Auditorium
Chair: Kelleher Neil, Northwestern University, USA
04:00 pm

Bridged MS Imaging of Proteins and Top-Down Spatially-Resolved Proteomics Strategy to better Characterize Tumor Microenvironment

I. Fournier
University of Lille, France 
04:30 pm

An isotope depletion strategy for improved top-down fragmentation analysis.

K. Gallagher1, M. Palasser2, S. Hughes1, C. L. Mackay1, D. P. A. Kilgour3, D. J. Clarke1
1University of Edinburgh, Edinburgh, United Kingdom 2Universität Innsbruck Innrain, Institut für Organische Chemie and Center for Molecular Biosciences Innsbruck (CMBI), Innsbruck, Austria 3Chemistry and Forensics, Rosalind Franklin Building, Nottingham Trent University, Nottingham, United Kingdom 
04:50 pm

Tandem Mass Spectrometry using Electron Capture Dissociation for the Structural Characterization of Intact Proteins

J. Williams1, C. Hughes1, J. Brown1, J. Beckman2, V. Voinov2
1Waters Corporation, Wilmslow, United Kingdom 2e-MSion, Corvallis, USA 
05:10 pm

And now a word from our sponsors!

05:20 pm

Round table: Future directions of top-down proteomics

J. Loo1, N. Kelleher2
1University of California, USA 2Northwestern University, USA 
08:00 pm

Cruise Dinner on the Seine River

Thursday, February 14, 2019
Native Top-Down and Beyond…
09:00 am-01:15 pm
CIS Auditorium
Chairs: Loo Joseph, University of California Los Angeles, USA - Chamot-Rooke Julia, Institut Pasteur, France
09:00 am

Native MS Coupled to Surface Collisions for "Complex-Down" Dissociation

V. Wysocki
Ohio State University, USA 
09:50 am

In-depth Investigation of recombinant proteins in crude samples

G. Ben-Nissan, S. Vimer, J. Cveticanin, M. Sharon
Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel 
10:20 am

Fast-photochemical Oxidation of Proteins and Top-down Mass Spectrometry

G. Yassaghi1, Z. Kukacka1, P. Pompach1-2, D. Fabris1-3, P. Novak1-2
1Laboratory of Structural Biology and Cell Signaling, Institute of Microbiology, Prague, Czech Republic 2Department of Biochemistry, Charles University, Prague, Czech Republic 3RNA Institute, The State University of New York, Albany, USA 
10:40 am

And now a word from our sponsors!

10:50 am


11:20 am

Mass and charge distributions of entire amyloid fibers by charge detection mass spectrometry: mapping heterogeneity, polymorphism and co-aggregation

R. Antoine
University of Lyon, France 
11:50 am

Nanomechanical resonators based neutral MS of viral capsids above 100 MDa

S. Dominguez-Medina1, S. Fostner2, M. Defoort2, M. Sansa2, A.K. Stark1, M. Halim1, E. Vernhed3, M. Gely2, G. Jourdan2, T. Alava2, P. Boulanger3, S. Hentz2, C. Masselon1
1EDYP Laboratory, CEA Grenoble, Grenoble, France 2LETI, CEA Grenoble, Grenoble, France 3I2BC, CNRS, Orsay, France 
12:10 pm

Closing lecture: Membrane Proteins – the Lipid Connection

C. Robinson
University of Oxford, United Kingdom 
01:00 pm

Final remarks

J. Chamot-Rooke, P. Danis


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